Fluoxetine Action on Murine T-Lymphocyte Proliferation: Participation of PKC Activation and Calcium Mobilisation

VALERIA AYELLI-EDGAR; ANA MARÍA GENARO; GRACIELA ALICIA CREMASCHI; LEONOR STERIN-BORDA

CELLULAR SIGNALLING

ELSEVIER SCIENCE INC

Lugar: Oxford; Año: 1998 vol. 10 p. 721 - 726

0898-6568

The present study was undertaken to analyse the effect of fluoxetine upon murine T-lymphocyteproliferation. We found that fluoxetine exerted a dual effect, which depended on the degree of lymphocyte activation:at mitogenic concentration (2 mg/mL) of concavalin A (Con A), we observed an inhibitory effect oncellular proliferation, whereas, on submitogenic Con A concentration (1 mg/mL), fluoxetine stimulated the cellularresponse. Given these facts, we studied PKC activation and calcium mobilisation in both stimulatory and inhibitory effects of fluoxetine on T-cell proliferation. We observed that fluoxetine increased PKC translocation obtained with 1 mg/mL Con A concentration, whereas PKC was degraded when 2 mg/mL was used. This mechanism is thought to be mediated by calcium mobilisation. According to our results, fluoxetine seemed to modulatecalcium influx, which, in turn, would influence PKC translocation, modulating the immune response.