INVESTIGADORES
GENARO Ana Maria
artículos
Título:
Involvement of endogenous nitric oxide signalling system in brain muscarinic acetylcholine receptor activation
Autor/es:
TANIA BORDA; ANA MARÍA GENARO; LEONOR STERIN-BORDA; GRACIELA ALICIA CREMASCHI
Revista:
JOURNAL OF NEURAL TRANSMISSION. GENERAL SECTION.
Editorial:
SPRINGER WIEN
Referencias:
Lugar: Viena; Año: 1998 vol. 105 p. 193 - 204
ISSN:
0300-9564
Resumen:
Biochemical signalling events coupled to muscarinic cholinergic
receptors (mAChR), specifically those related to nitric oxide (NO)
production, were studied on rat cerebral frontal cortex. The mAChR
agonist carbachol was found to exert a specific biphasic action on NO
synthase (NOS) activity: low doses ranging between 10(-9) M to 10(-7) M
lead to NOS activation while higher doses (>10(-6) M) inhibited
enzymatic activity. Carbachol stimulatory action was blunted by agents
that interfere with calcium-calmodulin while a protein kinase (PKC)
inhibitor, staurosporine was able to abrogate the inhibitory effect.
Moreover, PKC activity showed maximum translocation to cerebral frontal
cortex membranes with carbachol concentrations that inhibited NO
production. Products from phosphoinosite (PI) hydrolysis are involved in
these actions as carbachol was found to increase PI turnover in a dose
dependent manner. These results would serve as an example of cross-talk
between both enzymatic pathways.