INVESTIGADORES
ISLA maria ines
artículos
Título:
Comparative study of antioxidant and anti-inflammatory activities and genotoxicity of alcoholic and aqueous extracts of four Fabiana species that grow in mountainous area of Argentina
Autor/es:
CUELLO S, ALBERTO MR, ZAMPINI IC, ORDOÑEZ RM, ISLA MI
Revista:
JOURNAL OF ETHNOPHARMACOLOGY
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Año: 2011 vol. 137 p. 512 - 522
ISSN:
0378-8741
Resumen:
Ethnopharmacological relevance: Fabiana species (Solanaceae family) extracts have long been used in
Argentinean traditional medicine as anti-inflammatories, antiseptic, bone fractures and others diseases,
but there is no scientific evidence which supports their use.
Aim of the study: The present study was conducted to evaluate the ability of aqueous and ethanolic extracts
of four Fabiana species (Fabiana bryoides Phil., Fabiana punensis A.C. Arroyo, Fabiana densa J. Rèmy and
Fabiana patagonica Speg.) to inhibit key enzymes in inflammatory processes, free radical scavenging
properties and genotoxic effects.
Materials and methods: HPLC-DAD of aqueous and ethanolic extracts from four Fabiana species was
established. All Fabiana extracts were evaluated on their ability to inhibit hyaluronidase and lipoxygenase
enzymes to assess their activity against inflammatory mediators. Antioxidant capacity was
determined using the 2,2-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the 1,1-diphenyl-
2-picrylhydrazyl (DPPH) scavenging assays and -carotene-linolenic acid assay. Genotoxicity was
evaluated by the Ames assay.
Results: The results indicated that the chromatographic patterns of four Fabiana species were different in
quantity and absorption intensity of peaks. The alcoholic extract of Fabiana punensis was the most active
scavenger of DPPH? and ABTS?+ radicals (SC50 values of 3.85
±
0.24 and 2.56
±
0.10 g GAE/mL, respectively).
Fabiana patagonica extracts exhibited the highest peroxyl radical scavenging activity compared
with the other three taxa (IC50 values between 1.00
±
0.04 and 4.46
±
0.40 g GAE/mL for all extracts)
and anti-lipoxygenase activity with IC50 values between 12.5 and 15.5 g GAE/mL. The absence of mutagenicity
indicates that the DNA does not seem to be a relevant target for these extracts. Fabiana bryoides
ethanolic extract showed an interesting effect: it inhibited spontaneous mutagenesis, which could be
considered as an antimutagenic effect in the TA98 (+S9) and TA100 (+S9/−S9) strains. The potency differences
found between the species could be consequence of the different phytochemical pattern observed
by HPLC.
Conclusions: The inhibitory effects on lipoxygenase and hyaluronidase, free radical scavenging activities
and lack of genotoxicity of Fabiana extracts may support the folk use of Fabiana punensis, Fabiana
patagonica, Fabiana bryoides and Fabiana densa as inhibitor of inflammatory mediators.