Hydroxylated jasmonate levels during stolon to tuber transition in Solanum tuberosum L.

CENZANO A, VIGLIOCCO A, MIERSCH O, ABDALA G.

Potato Research

European Association for Potato Research

Lugar: Wageningen, Holanda; Año: 2005 vol. 1 p. 107 - 115

0014-3065

Various octadecanoids and derived compounds have been identified in potato plant leaves. However, little information exists regarding the isolation of the jasmonate hydroxylated forms in stolons or tubers. We investigated the endogenous jasmonate variations in the apical region and stolons of Solanum tuberosum cv. Spunta, during the transition of stolons into tubers. Apical hooked stolon, swelling stolon initiation, advanced swelling stolon and tuber were collected from plants of 8 weeks of age. The material was cut into apical region and stolon for jasmonate analysis. We identified jasmonic acid (JA), methyl jasmonate (Me-JA), 11-OH-JA, 12-OH-JA and also 12-oxo-phytodienoic acid (OPDA) and a conjugate (JA-Ile). The content of JA and 12-OH-JA decreased in the apical region and maintained at a constant high level in stolon along the stages of tuber formation. This fact indicates that the apical region might be a site of JAs-utilization or metabolization and stolon could act as supplying pathways of JAs to the apical region. The content of 12-OH-JA was higher than that of 11-OH-JA in the four stages analyzed, both in the apical regions and in stolons. However, these compounds showed a different time-course in the apical region: while 11-OH-JA increased, 12-OH-JA content decreased. Thus, JA from leaves or roots could be transported as 12-OH-JA to the apical region, stimulating tuber formation by cell expansion and vascular tissue differentiation.