MALCHIODI Emilio Luis
In vitro antiprotozoal activity and chemical composition of Ambrosia tenuifolia and A. scabra essential oils
SÜLSEN VP, CAZORLA SI, FRANK FM, DI LEO LIRA PMR, ANESINI CA, GUTIERREZ YAPU D, GIMÉNEZTURBA A, BANDONI AL, MALCHIODI EL, MUSCHIETTI LV, MARTINO VS
NATURAL PRODUCT COMMUNICATIONS
NATURAL PRODUCTS INC
Lugar: Westerville; Año: 2008 vol. 3 p. 557 - 562
The in vitro antimalarial, trypanocidal and leishmanicidal activities and the chemical composition of Ambrosia tenuifolia and A. scabra essential oils were investigated. The oils, obtained by hydrodistillation of the aerial parts, showed significant antimalarial activity against the chloroquine sensitive (F32) and the chloroquine resistant (W2) strains of Plasmodium falciparum with 50% inhibitory concentration (IC50) values ranged from 2.8 to 7 ìg/mL. Against Trypanosoma cruzi epimastigotes, only A. tenuifolia exerted slight activity (IC50 = 59.7 ìg/mL). None of the essential oils showed toxicity towards Leishmania mexicana. Cytotoxicity for murine T-lymphocytes was compared with the parasiticidal activity by using the selectivity index (SI) ratio. The chemical composition of A. tenuifolia and A. scabra essential oils was analyzed by GC-FID-MS. Thirty-eight and twenty-four compounds were identified in A. tenuifolia and A. scabra, respectively. The major constituents of A. tenuifolia oil were germacrene D (22.9%) and bicyclogermacrene (14.2%), while the oil of A. scabra contained limonene (22.0%), caryophyllene oxide (13.8%) and (E)-caryophyllene (13.5%). This is the first time that the antiprotozoal activity of both oils and the chemical composition of A. scabra essential oil have been reported.