Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex

AVILA HG; MOZZONI C; TRANGONI MD; CRAVERO SLP; PÉREZ VM; VALENZUELA F; GERTISER ML; BUTTI MJ; KAMENETZKY L; JENSEN O; ROSENZVIT M C

VETERINARY PARASITOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2020

0304-4017

Cystic echinococcosis represents a significant problem in human and animal health and constitutes one of themost severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent isthe complex Echinococcus granulosus sensu lato (s. l.), composed of several species/genotypes. Diagnosis in thedefinitive host and molecular epidemiology studies are important points for cystic echinococcosis control. Herewe developed a new copro-LAMP assay, LAMP EGSL, for diagnosis in the definitive host for simultaneous detectionof Echinococcus granulosus sensu stricto (s. s.), Echinococcus ortleppi, and Echinococcus canadensis species.Also, the analytical sensitivity, specificity and plausibility of performance in a rural context of a previouslyreported species-specific LAMP reaction, was evaluated. Both reactions showed high analytical sensitivity values(10 fg-100 fg DNA) and did not show cross reaction with DNA from host or other helminthic parasites. LAMPEGSL was performed with samples from an endemic area. In addition, the alkaline hydrolysis of one E. granulosuss. s. adult parasite followed by specific LAMP to E. granulosus s. s. was performed in a laboratory with lowresources from another cystic echinococcosis endemic area. The results obtained suggest that LAMP EGSL representsa potential tool for canine diagnosis that could be useful for cystic echinococcosis control programs. Inaddition, we showed that LAMP reaction for E. granulous s. s., E. ortleppi and E. canadensis specific detection,could be useful for molecular epidemiology studies applicable to the definitive host. Both reactions were performedin endemic, rural areas without sophisticated equipment.