INVESTIGADORES
MUGLIA Cecilia Isabel
congresos y reuniones científicas
Título:
Analysis of Intestinal Stem Cells in Inflammatory Pathologies
Autor/es:
POLO, B; BARBIERA, E; MENENDEZ, L; BOROBIA P; ZUBIRI, C; ZOSI, A; GUZMAN, L; BERNEDO, V; ALTAMIRANO E; CORREA, G; YANTORNO, M; ROCCA, A; MUGLIA C.I.; DOCENA, G.
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Conjunta SAIC SAI FACIC SAFIS 2022; 2022
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Intestinal epithelial cells (IECs) are key elements of the intestinalepithelial compartment. It is implicated in cross-talk with the microbiotaand the underlying immune cells and in barrier integrity. A continuousreplacement of differentiated cells maintains the homeostasisof the intestinal epithelium through the replication of stem cells(ISCs) located within the crypts. However, in different inflammatorysettings, the renewal of epithelial cells may be altered, contributingto different chronic pathologies. This work aimed to identify andquantify human intestinal stem cell populations in different pathologicalconditions. We quantified stem cells in intestinal biopsies (23)and surgical pieces (8) of inflamed and surrounding tissue of adultpatients with colorectal cancer (CCR), inflammatory bowel diseases(IBD) (19), and healthy controls (HC) (8), and from colorectal polypsof pediatric patients sensitized to cow´s milk protein (11) and biopsiessurrounding the polyps (3). Stem cells were identified and quantifiedas LGR5+ cells by flow cytometry and confocal microscopy.Our results showed a higher frequency of LGR5+ cells in inflamedIBD tissue compared to non-inflamed IBD tissue and gut from HC(p=0,005). In contrast, we found a smaller fraction of LGR5+ stemcells in polyps samples compared to its control surrounding biopsies(p=0,03). Immunofluorescence microscopy revealed the presenceof LGR5+ cells within the epithelial compartment, and mainly locatedin the crypts. In conclusion, we found an expansion of colonicstem cells in different pathologies and inflammatory conditions, beingmore expanded in inflamed tissues. Further analysis, includingfunctional and next-generation sequencing assays, is in progress toget more insights into the role of these cells in the different inflammatorypathologies.