Lipase production by Candida rugosa

BERTOLUZZO SM; LÓPEZ D; BIASOLI M; BERTOLUZZO MG

Mendoza

Encuentro; 28° Reunión Cientíifca Anual Sociedad de Biología de Cuyo; 2010

Sociedad de Biología de Cuyo

The use and preparation of the main chiral drugs as single enantiomers is one of the most important achievements in pharmaceutical science. For chiral drugs, opposite enantioforms act with different biological properties and the distomer could give undesirable effects. Ibuprofen is one of the most important drugs of the classes of drugs non-steroidal anti-inflammatory, used as a racemate in pharmaceutical formulations, but have their pharmacological activity mainly in the form enantiomeric S (+).The aim of this work is to produce lipase from Candida rugosa yeast strains for later use in the resolution of ibuprofen. Cultures were performed in 500 ml Erlenmeyer flasks with 120 ml of culture medium were inoculated with 1 ml of spore suspension and incubated for 72 h at 35 ° C on a rotary shaker. After this period, the cultures were filtered and treated with ammonium sulfate (80% saturation). The precipitates were dialyzed for 24 h against sodium phosphate buffer, pH 7.0 and lyophilized for later use as a powder lipase. The hydrolytic activity of the biocatalysts was determined by measuring the free acid of a sample of olive oil in the presence and absence of lipases. Therefore, a sample of olive oil was dissolved in a mixture of alcohol and ether and titrated with Na(OH) 0.1 N using phenolphthalein as indicator. The free acid obtained was 6 times higher than the acidity of the sample without lipase. The protein concentration was found to be 2 mg / ml using the Biuret method.