Optimization of variables in photodynamic antifungal assays against Candida species Optimización de variables en ensayos de actividad antifúngica fotodinámica frente a especies de Candida

BULACIO, LUCÍA; RAMADÁN, SILVANA; DALMASO, HERNÁN; LUQUE, ALICIA; SORTINO, MAXIMILIANO

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Año: 2021

0325-7541

Oral infections due to yeasts of the genus Candida are very common in patients with predisposing factors, such as physiological conditions or certain underlying diseases. Moreover, oral candidiasis can also evolve into disseminated forms. In this work, strains of the genus Candida were studied to establish the optimal radiation conditions for photosensitizing compounds, in a photodynamic antifungal therapy protocol. A total of 39 isolates were evaluated. The strains were exposed to twelve dyestuffs, eight radiation sources and three different exposure times. Orthotoluidine blue exhibited good photodynamic activity, in combination with exposure to a 20 W reflector lamp LED (light-emitting diode) light for 60 minutes. When considering the difficulties of using conventional antifungal drugs, the emergence of resistant strains, recurrences, and adverse reactions of certain commonly used drugs, the photodynamic antifungal therapy is a promising strategy for the treatment of localized infections.