INVESTIGADORES
CARRIQUIRIBORDE Pedro
congresos y reuniones científicas
Título:
Global metabolic response in the bile of horned frog (Ceratophrys cranwelli) tadpoles exposed to endosulfan.
Autor/es:
MARINO, D.J.; SY, V.; NATALE, G.S.; RONCO, A.E.; CARRIQUIRIBORDE, P.
Lugar:
Boston
Reunión:
Congreso; SETAC North America 32th Annual Meeting; 2011
Institución organizadora:
Society of Environmental Toxicology and Chemistry (SETAC)
Resumen:
The global metabolic response in the bile of horned frog (Ceratophrys cranwelli) tadpoles exposed to endosulfan was assessed using UPLC-MS/MS. Tadpoles were exposed to 0, 5 and 10 µg/L of endosulfan (a.i.) during 96 h. Treatments were performed by triplicate exposing seven organisms per replica. The gallbladders from five tadpoles per replica were pooled in a microtube with 1 ml of nanopure water, centrifuged and filtered through 0.22 µm. Samples were analyzed using an UPLC system (ACQUITY UPLC®) with a C18 column (ACQUITY BEH) and couple to a tandem quadrupole mass spectrometer (Quattro Premier XE) equipped with an electrospray atmospheric pressure ionization interface (ZSpray). Data were acquired using the MassLynx software and processed for peak detection, matching and alignment using the XCMS open access software. The highest number of ions was achieved in ESI negative mode (1122 peaks). The number of ions 10-fold up and down regulated in tadpoles exposed to 5 µg/L, respect to the controls, was 64 and 59, respectively. In tadpoles exposed to 10 µg/L the number of peaks was 90 and 57, respectively. Most of these ions belong to the same time clusters, suggesting they were fragments of the same metabolites. The strongest down regulation was observed for a peak at m/z 367, showing a clear concentration dependent response with 7,684 and 15,311 fold reduction at 5 and 10 µg/L, respectively. The highest up regulation was observed for a peak at m/z 803, showing a concentration dependent response with a mean abundance of 0, 71 and 10,843 in 0, 5, and 10 µg/L, respectively. A characteristic response at the global metabolic profile was observed in the bile of C. cranwelli exposed to endosulfan, which could be useful as fingerprint of the exposure to this organochlorine. In addition, further identification of major responding compounds could help to gain insight on the toxic mechanisms of the insecticide.
