MIRANDA leandro Andres
congresos y reuniones científicas
GÁRRIZ A, MENÉNDEZ-HELMAN R, MIRANDA LA. Effects Of Estradiol and/or Ethynylestradiol on Sperm Quality, Fertilization, Embryo and Larval Survival of Pejerrey Fish (Odontesthes bonariensis). Latin America 11th Biennial Meeting Buenos Aires 7
Buenos Aires
Congreso; SETAC Latin America 11th Biennial Meeting; 2015
Institución organizadora:
17β-Estradiol (E2) and synthetic 17α-Ethynylestradiol (EE2) are estrogenic compounds present in surfacewaters as the consequence of municipal sewage discharges. The aim of this studywas to evaluate the effects of E2, EE2 and its mixtures on differentreproductive parameters and embryo-larval survival in pejerrey fish (Odontesthes bonariensis). In order toanalyze the effect of these compounds on sperm quality, fertilization %,embryo-larval survival (%), and the point of no return (PNR), different assayswere performed using concentrations 175, 350, 700 and 1400 ng/L of E2; 22.5,45, 90 and 180 ng/L of EE2; and mixtures M1 (175 E2 + 22.5 EE2, ng/L), M2 (350E2 + 45 EE2, ng/L), M3 (700 E2 + 90 EE2, ng/L) and M4 (1400 E2 + 180 EE2 ng/L).No significant differences in motility parameters were observed between E2 andEE2 treatments with the control group. However, significant decreases inmotility % were recorded for all the mixtures tested compared with controlsamples. For fertilization %, only the sperm activated with M4 showed asignificant decrease compared with the control group. In the case of embryosurvival there was only a significant decrease in the highest concentration ofEE2 compare with the control group. For the mixtures, M3 is the one that hadthe most adverse effect on embryo survival. In larval survival, there was asignificant decrease in concentration 175 and 700 ng/L of E2 compare with thecontrol group. In EE2 treatments, the ones with a significant reduction in thelarval survival were concentration 45 and 90ng/L. And for the mixture treatments,M1 M3 and M4 had a significantly lower larval survival than the control group.The PNR was only significantly different in M1 compared with the control group.The results obtained demonstrated that the exposure to mixtures of E2 and EE2affected fish sperm motility, fertilization % and, embryo and larval survival,even at relevant environmental concentrations, and highlight the need toconsider the effects of pollutants mixtures in ecotoxicological studies.