ALPHA-HEMOLYSIN INDUCED HUMAN ERYTHRO- CYTES ADHESION TO VASCULAR ENDOTHELIUM. A BIOMIMETIC APPROACH.

SAFFIOTI, NICOLÁS ANDRÉS*; LEAL DENIS, MARÍA FLORENCIA*; ALVAREZ, CORA LILIA; HERLAX, VANESA; SCHWARZBAUM, PABLO JULIO; PALLAROLA, DIEGO

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020; 2020

The α-haemolysin (HlyA) is an exotoxin produced by several strains of uropathogenic E. coli, one of the most important etiological agents of urinary infections. HlyA irreversibly binds to human erythrocytes (RBCs), initiating a degenerative process called eryptosis, characterized by Biochemical and morphological changes such as phosphatidylserine (PS) exposure to the external layer of the plasma membrane of RBCs, shrinkage, and swelling. HlyA-induced PS externalization can lead to adhesion of RBCs to vascular endothelial cells (VECs).We studied the capacity of HlyA-treated RBCs to adhere to: 1- activated endothelial HMEC-1 cells under different flow conditions (dynamic adhesion); 2- surfaces homogeneously covered with extracellular matrix components in static conditions. Results showed that HlyA induced adhesion of RBCs to VECs at low flow (0.2 dyn/cm2), although higher flows induced rapid detachment. On the other hand, HlyA treatment also induced static adhesion of RBCs to collagen or fibrinogen. Thus, HlyA-treated RBCs displayed high but weak adherence to VECs under the experimental conditions.Additionally, to study the molecular mechanism of the HlyA-induced adhesion of RBCs we designed a biomimetic device to emulate the conditions of the blood vessels.The device was built by coupling a microfluidic chip to a nanopatterned surface (NPS) coated with gold nanoparticles (AuNPs). Different adhesion molecules from the VECs could be anchored to theAuNPs to mimic exposure of adhesion molecules of an activated endothelium whereas, the architecture of the capillaries was emulated by a network of microfluidic channels built in polydimethylsiloxane (PDMS). The synthesis of the device was optimized by followingthe process with quartz microbalance and fluorescence microscopy.Future experiments using the device will allow investigating HlyA-induced adhesion of RBCs to specific adhesion molecules under flow conditions.