Genomic approach targeted to the genetic determinants of tomato fruit quality

KAMENETZKY L.; ASIS R.; BASSI S.; DE GODOY, F.; BERMÚDEZ L.; VAN SLUYS M.A.; FERNIE A. R.; GIOVANNONI J.; CARRARI F.

Cologne Alemania

Workshop; The 5th Solanaceae Genome Workshop; 2008

The adoption of tomato varieties selected for their high performance in terms of harvest indexhas considerably reduced accessibility to genetic basis of the Solanum genus. This fact has negatively impactedon the production of metabolites associated to the nutritional, flavor and pharmacological quality in tomato fruit.The main objective of our work is to determine the genetic determinants of key points regulating tomato fruitmetabolism. In particular, this presentation conveys on the analysis of 5 regions of the Solanum pennellii genomespanning metabolic QTL previously mapped on an introgression line population (S. lycopersicum x S. pennellii).Two genomic libraries from S. pennellii were screened with 143 overgo designed on the same number of mappedmarkers. Two type of markers were used, the first based on information available on the Solanaceae GenomeNetwork (www.sgn.cornell.edu) and the others were design on the bases of sequence information and furtherbioinformatic analyses of EST and BAC clones from S. lycopersicum genome. End-sequence analyzes of 345 BAC/COS anchored clones including gene annotation; contigs assembling and comparison with the orthologous regionof S. lycopersicum were used to build physical maps of these regions. Fulllength sequences of selected clonescontaining candidate genes associated with the observed metabolic traits are being generated. The results obtainedhere allow the identification of wild alleles potentially involved in the tomato fruit quality determination.