Influence of the nature of the support in the catalytic performance of CALB: experimental and theoretical evidences

CARLA JOSE; M.V TOLEDO; PAULA NICOLÁS; LASSALLE, VERÓNICA; M.L.FERREIRA; LAURA BRIAND

Catal. Sci. Technol.

Royal Society of Chemistry

Año: 2018

2044-4761

The immobilization of the lipase B of Candida antarctica (CALB) was carried out onvarious hydrophilic and hydrophobic supports through adsorption and covalent bondinteractions. The catalytic performance of the biocatalysts was evaluated in theenantioselective esterification the R/S-ibuprofen with ethanol without co-solventsadded. The present investigation demonstrates that the biocatalysts based onCALB immobilized by adsorption on hydrophobic supports (acrylic resin,polymethylmethacrylate, polystyrene, epoxy resin and polypropylene) possesshigher conversion of ibuprofen and enantiomeric excess towards the S-enantiomerthan CALB immobilized onto hydrophilic materials. Additionally, the enzyme loadingis a key factor influencing the conversion of the substrate regardless the nature ofthe support material. High enzyme loading is detrimental for the specific activity ofthe biocatalysts. Experimental and molecular modeling investigations providedevidences of the influence of the water activity in the stereospecificity of the lipase.