KOCHEN Sara Silvia
congresos y reuniones científicas
The non-dysplastic temporal pole in patients with drug resistant temporal lobe epilepsy and hippocampal sclerosis: neuropathopathology and clinical aspects
SANZO M; KONOPTKA H; GONZALEZ STIVALA E; KOCHEN S
Congreso; Word Epilepsy Congress; 2019
Purpose: The temporal pole plays an important role inthe genesis and propagation of seizures in temporal lobe epilepsy (TLE),however the mechanisms involved are not fully understood. The aim of this studywas to determine the expression pattern and the clinical correlations ofhistopathological markers (calbindin inmunoreactivity, GFAP and NeuN) in thenon-dysplasic temporal polar cortex resected during surgical procedure ofpatients with temporal lobe epilepsy (TLE) and hippocampal sclerosis (HS). Methods: In all patients, clinical, neurological, EEG, video-EEG, MRI,psychiatric and neuropsychological evaluation has been performed prior tosurgery. Patients with cortical dysplastic alterations detected by MRI or byhistopathology (Blümke et al. criteria) were excluded. Microtome histologicalsections from the polar temporal cortex were processed by immunohistochemistrywith anti-NeuN, anti-calbindin antibodies (CB) and anti-acid gliofibrillaryprotein (GFAP). A semiquantitative analysis of the immunoreactivity was carriedout using Image J software. The Student´s Test/Chi Square Test and Pearsoncorrelations were applied. Results: 27 patients (14 women and 13 men, age 32.04 ± 9.53 years) and 5post-mortem controls were included. The mean time of epilepsy duration was21,85 ±10.6 years, the mean age of epilepsy onset was 9.63 ±7.9 years (16patients had right HS (59.25%) and 11 patients had left HS (40.74%). Asignificant increase in the expression of GFAP and in CB were found(p <0.05) compared to the controls. A higher percentage of CB+ cells in layer II,and increase in the CB reactive area in layer II and layer III (p < 0.05). Conclusions: In this exploratory study an increase in the reactivegliosis and in the expression of CB (a marker of GABAergic interneurons) werefound. Both alterations would be triggered by the epileptic activity in thepolar temporal cortex. The prognostic implications implications of thesefeatures will be study in future approaches.