Doublecortin (DCX) immunoreactivity in hippocampus of chronic refractory temporal lobe epilepsy patients with hippocampal sclerosis

LÓPEZ-COSTA JJ, D´ALESSIO L, KONOPKA H, LÓPEZ EM, SEOANE E, CONSALVO D, ODDO S, SILVIA KOCHEN

SEIZURE : THE JOURNAL OF THE BRITISH EPILEPSY ASSOCIATION.

W B SAUNDERS CO LTD

Año: 2010 vol. 19 p. 567 - 572

1059-1311

Introduction: Status epilepticus increases the production of new neurons (hippocampal neurogenesis) and promotes aberrant migration. However chronic experimental models of epilepsy and studies performed in human epilepsy showed controversial results suggesting a reduction in hippocampal neurogenesis in late stages of the disease. Doublecortin (DCX) has been validated to determine alterations in the production of new neurons in the human hippocampus. Objectives Determine DCX expression in human hippocampal sclerosis (HS) from patients who underwent epilepsy surgery forrefractory temporal lobe epilepsy (TLE). Methods Hippocampal sections of 9 patients with HS and TLE who underwent surgery, were processed using immunoperoxidase forDCX. Archival material from 5 normal post-mortem hippocampus were simultaneously processed. Results Significantly lower staining intensity was observed in DCX-positive neurons localized in dentate gyrus (DG) and in CA1 ofepileptic hippocampus; lower DCX reactive area was observed in pyramidal layers of CA1; and a reduced in the mean number of DCX-positive neurons were determined in DG compared to normal hippocampus (p < 0.05). Conclusions This study found a decrease in DCX expression in hippocampus of patients with HS and chronic and refractory TLE suggesting alterations in NG and hippocampal synaptogenesis with potential cognitive and emotional repercussion.