PATHOGEN-SPECIFIC T CELLS FROM PATIENTS WITH INFLAMMATORY BOWEL DISEASES CAN BE MODULATED BY PROBIOTICS FROM KEFIR

RENATA CURCIARELLO; SANTIAGO BAYER; GUILLERMO DOCENA; AUGUSTO VILLAVERDE; FRANCISCO TUFARÉ; LAURA GARBI; GUSTAVO CORREA; MARTIN YANTORNO; ALICIA SAMBUELLI; ANDRES ROCCA; KARINA CANZIANI; AYELEN HUGO; CECILIA ALVAREZ; MARTÍN RUMBO; AGUSTINA ERREA; MARÍA DE LOS ANGELES SERRADELL; EMANUEL BARBIERA; ILEANA SALTO; CECILIA MUGLIA

Mar del Plata

Congreso; Reunión Conjunta de Sociedades de BioCiencias; 2018

SAI SAFIS SAIC

Kefir is a fermented milk with health-promoting properties. Lamina propria T cells (LPTC) play a central role in the pathogenesis of In- flammatory Bowel Diseases (IBD). These cells contribute to muco- sal inflammation by secreting pro-inflammatory cytokines and being resistant to apoptosis. We aimed to modulate the proliferation and the pro-inflammatory response of pathogen-specific LPTC from IBD patients using microorganisms from kefir.Colonic biopsies from IBD patients were incubated with probiotics from kefir and cytokines in supernatants were evaluated by ELISA afterwards. LPTC were isolated from biopsies (N=23; 7 from Crohn's Disease patients-CD and 15 from ulcerative colitis-UC patients) by collagenolytic digestion. In order to expand pathogen-specific T lym- phocytes, cells were cultured with enteroadhesive (EA) Escherichia coli extract and IL-2 for 10 days. Thereafter, LPTC were incubated with anti-CD3/anti-CD28 and with microorganisms from kefir (En- terococcus durans and Lactobacillus kefiri), their conditioned media or 10 µM lactate for 96 hs. LPTCs proliferation (CFSE) and TNF-a, IFN-y, IL-6, IL-10 and IL-13 cytokine secretion were evaluated (ELI- SA).We found that E. durans and L. kefiri diminished the pro-inflamma- tory cytokine production by inflamed tissues (IL-6: 1800±670 and 576±3 vs 6900±2000 pg/ml basal production, respectively; IL-8: 38000±11000 and 32000±7300 vs 80000±27000 pg/ml basal pro- duction, respectively). EA E. coli specific LPTC lines were developed from all patients. Cellular proliferation of activated CD4+/CFSE-/PI- LPTC decreased significantly with L. kefiri , E. durans and lactate (P