BECAS
BARBIERA ROMERO Emanuel
congresos y reuniones científicas
Título:
Micro-RNA 21 but not miR-155 is differentially expressed in the gut mucosa of patients with intestinal inflammatory conditions
Autor/es:
EMANUEL BARBIERA ROMERO; MARÍA BELÉN POLO; MALENA FERREYRA
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión conjunta de Biociencias SAI SAIC SAFIS; 2022
Institución organizadora:
SAI SAIC SAFIS
Resumen:
Mucosal myofibroblasts are key stromal cells involved in IBD pathogenesis and in the CRC tumor micro-environment. Here, we aimed to study the expression of miR-21 and miR-155 in the mucosa and intestinal fibroblasts from IBD and CRC patients, as potential biomarkers to predict CRC outcome in patients with chronic intestinal inflammatory disorders. Target genes and proteins regulated by these miRNAs were also analyzed.Total RNA was obtained from mucosal explants from IBD(n=25), polyp(n=8), CRC(n=7), and healthy control (HC) patients (n=16). Intestinal fibroblast primary cultures were established from colon surgical pieces (n=10). Microvesicles were obtained from fibroblast culture supernatant by ultracentrifugation. MiR-21, miR-155, PDCD4, CBX7, KRAS transcript expression was quantified by qPCR in mucosal and cell samples. Also, TNF-α was quantified by ELISA while FAP (Fibroblast Activation Protein) expression was evaluated by immunofluorescence in biopsies. We found that miR-21 was highly expressed in inflamed biopsies compared to uninflamed mucosa (p
