IL-13 promoted the phosphorylation of STAT3 and STAT6 on colonic epithelial cells with the secretion of TSLP and CCL26

JULIÁN VACCARO; KARINA EVA CANZIANI; LUCIANA GUZMAN; VIVIANA BERNEDO; MARCELA GARCÍA; EUGENIA MARGARITA ALTAMIRANO; RENATA CURCIARELLO; CECILIA MUGLIA; GUILLERMO DOCENA

Bueno Aires

Congreso; Reunión Conjunta SAIC. SAI. AAFE. NANOMED-AR; 2021

SAI, SAIC, AAFE, NANOMED.AR

Food allergy prevalence is increasing worldwide. Eosinophilic inflammatory responses can be associated with food allergy and the underlying mechanisms in human colonic mucosa are poorly understood. We previously reported that allergic sensitized pediatric patients with juvenile polyps showed local IgE class-switch recombination with high levels of TSLP, IL-33 and CCL26 (main chemoattractant for eosinophils), that might be related with the hypereosinophilia. This study aimed to investigate the intestinal epithelial cells as a source for these soluble mediators under different inflammatory settings.Caco-2 cells were stimulated with IL-13 (10 ng/ml) or IFN-γ (10 ng/ml) and CCL26, TSLP and IL-33 were evaluated by ELISA in culture supernatants. Cell activation was analyzed with a tyrosine-kinase phosphorylation array and finally, kinase activation was confirmed by immunoblotting. Comparison between groups was made using Student´s t-test.We found that TSLP and CCL26 levels were significantly increased in Caco-2 cells in response to IL-13 (p