INVESTIGADORES
SAMBETH jorge Enrique
artículos
Título:
Biosynthesis of anti-HCV compounds using thermophilic microorganisms
Autor/es:
RIVERO CINTIA; DE BENEDETTI ELIANA; JORGE SAMBETH; TRELLES JORGE; LOZANO MARIO
Revista:
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2012 vol. 22 p. 6059 - 6062
ISSN:
0960-894X
Resumen:
This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-20-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6- chloropurine-20-deoxyriboside. The obtained compounds can be used as antiviral agents.
