Preconditioning donor with a combination of tacrolimus and rapamacyn to decrease ischaemia-reperfusion injury in a rat syngenic kidney transplantation model

CICORA, F.; ROBERTI, J.; VASQUEZ, D.; GUERRIERI, D.; LAUSADA, N.; CICORA, P.; PALTI, G.; CHULUYAN, E.; GONZALEZ, P.; STRINGA, P.; RAIMONDI, C.

CLINICAL AND EXPERIMENTAL IMMUNOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2012 vol. 167 p. 169 - 177

0009-9104

Reperfusion injury remains one of the major problems in transplantation. Repair from ischaemic acute renal failure (ARF) involves stimulation of tubular epithelial cell proliferation. The aim of this exploratory study was to evaluate the effects of preconditioning donor animals with rapamycin and tacrolimus to prevent ischaemia-reperfusion (I/R) injury. Twelve hours before nephrectomy, the donor animals received immunosuppressive drugs. The animals were divided into four groups, as follows: group 1 control: no treatment; group 2: rapamycin (2mg/kg); group 3 FK506 (0, 3mg/kg); and group 4: FK506 (0, 3mg/kg) plus rapamycin (2mg/kg). The left kidney was removed and after 3h of cold ischaemia, the graft was transplanted. Twenty-four hours after transplant, the kidney was recovered for histological analysis and cytokine expression. Preconditioning treatment with rapamycin or tacrolimus significantly reduced blood urea nitrogen and creatinine compared with control [blood urea nitrogen (BUN): P