Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor

KAMEERBECK C.B; MATEOS M.V.; VALLES A.S.; PEDICONI M.F.; BARRANTES F.J; BORRONI M.V.

INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND CELLULAR BIOLOGY

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2016

1357-2725

Diacylglycerol (DAG), a second messenger involved in different cell signalingcascades, activates protein kinase C (PKC) and D (PKD), among other kinases.The present work analyzes the effects resulting from the alteration of DAGlevels on neuronal and muscle nicotinic acetylcholine receptor (AChR)distribution. We employ CHO-K1/A5 cells, expressing adult muscle-type AChRin a stable manner, and hippocampal neurons, which endogenously expressvarious subtypes of neuronal AChR. CHO-K1/A5 cells treated withdioctanoylglycerol (DOG) for different periods showed augmented AChR cellsurface levels at short incubation times (30 min-4 h) whereas at longer times(18 h) the AChR was shifted to intracellular compartments. Similarly, in cultured hippocampal neurons surface AChR levels increased as a result of DOGincubation for 4 h. Inhibition of endogenous DAG catabolism produced changesin AChR distribution similar to those induced by DOG treatment. Specificenzyme inhibitors and Western blot assays revealed that DAGs exert theireffect on AChR distribution through the modulation of the activity of classicalPKC (cPKC), novel PKC (nPKC) and PKD activity.