INVESTIGADORES
IRAZOQUI Fernando Jose
artículos
Título:
Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides
Autor/es:
MOYANO, AL; COMÍN, R; VILCAES, AA; ROTH, GA; IRAZOQUI FJ; NORES, GA
Revista:
GLYCOBIOLOGY
Editorial:
OXFORD UNIV PRESS INC
Referencias:
Lugar: Oxford; Año: 2012 vol. 22 p. 1768 - 1774
ISSN:
0959-6658
Resumen:
Immunization of rabbits with bovine brain gangliosides
induced an experimental neuropathy, with clinical signs
resembling GuillainBarré syndrome. All the immunized
animals developed immunoglobulin G immunoreactivity
to GM1 ganglioside. In a few (4 of 27) animals, an additional
anti-ganglioside antibody population showing an
unusual binding behavior was detected. Enzyme-linked
immunosorbent assay and thin-layer chromatography
immunostaining analyses showed that the binding of these
unusual antibodies required the presence of two co-localized
gangliosides. Maximal interaction was observed to a
mixture of GM1 and GD1b, but the antibodies also showed
animals developed immunoglobulin G immunoreactivity
to GM1 ganglioside. In a few (4 of 27) animals, an additional
anti-ganglioside antibody population showing an
unusual binding behavior was detected. Enzyme-linked
immunosorbent assay and thin-layer chromatography
immunostaining analyses showed that the binding of these
unusual antibodies required the presence of two co-localized
gangliosides. Maximal interaction was observed to a
mixture of GM1 and GD1b, but the antibodies also showed
Barré syndrome. All the immunized
animals developed immunoglobulin G immunoreactivity
to GM1 ganglioside. In a few (4 of 27) animals, an additional
anti-ganglioside antibody population showing an
unusual binding behavior was detected. Enzyme-linked
immunosorbent assay and thin-layer chromatography
immunostaining analyses showed that the binding of these
unusual antibodies required the presence of two co-localized
gangliosides. Maximal interaction was observed to a
mixture of GM1 and GD1b, but the antibodies also showed
density-dependent binding to GD1b. The antibodies were
purified by affinity chromatography and displayed the
ability to target antigens in biological membranes (rat
synaptosomes).
ability to target antigens in biological membranes (rat
synaptosomes).
purified by affinity chromatography and displayed the
ability to target antigens in biological membranes (rat
synaptosomes).
ability to target antigens in biological membranes (rat
synaptosomes).
density-dependent binding to GD1b. The antibodies were
purified by affinity chromatography and displayed the
ability to target antigens in biological membranes (rat
synaptosomes).
ability to target antigens in biological membranes (rat
synaptosomes).
fied by affinity chromatography and displayed the
ability to target antigens in biological membranes (rat
synaptosomes).