INVESTIGADORES
NERLI Bibiana Beatriz
artículos
Título:
Cloud point extraction based on non-ionic surfactants: an ecofriendly tool for recovering papain from papaya latex
Autor/es:
DI GIACOMO, MELISA; BERTONI, FERNANDO; ROCHA, MARÍA VICTORIA; NERLI, BIBIANA BEATRIZ; RODRIGUEZ, FERNANDA
Revista:
Journal of Environmental Chemical Engineering
Editorial:
Elsevier
Referencias:
Año: 2022
ISSN:
2213-3437
Resumen:
The potential use of cloud point extraction with aqueous micellar two-phase systems (AMTPS), formed by the non-ionic biodegradable surfactants Triton X-114 (TX-114) and Genapol X-080 (GX-080), to recover papain (PAP) from fresh papaya latex was evaluated. Binodal curves obtained for both surfactants, in absence and presence of PAP, showed cloud points below 40°C, the PAP presence slightly favoring the phase separation phenomenon.Partitioning experiments showed that PAP migrated toward the micelle-poor phase independently of the surfactant and the working temperature assayed, this behavior being consistent with this enzyme hydrophilic character. PAP recoveries of over 60% in the poor-micelle phase were obtained when partitioned fresh papaya latex in both surfactant-based AMTPS. TX-114 systems (4.00% w/w, 33°C) allowed recovering 66% of PAP with a purification factor of 4.6. On GX-080 systems (4.00% w/w, 42°C) a yield of 78% PAP and a purification factor of 3.7 were achieved. This AMTPS also allowed the separation of PAP from reducing sugars present in papaya latex.These results show that TX-114 and GX-080-based AMTPS could be potentially used as simple and economical tools at recovering PAP from papaya latex.