INVESTIGADORES
ORDEN Alejandro Agustin
artículos
Título:
Coumarin metabolic routes in Aspergillus spp.
Autor/es:
CELESTE AGUIRRE PRANZONI; ALEJANDRO A. ORDEN; FABRICIO R. BISOGNO; CARLOS ARDANAZ; CARLOS E. TONN; MARCELA KURINA-SANZ
Revista:
Fungal Biology
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Año: 2011 vol. 115 p. 245 - 252
ISSN:
1878-6146
Resumen:
Coumarin metabolism by several Aspergillus strains was studied. Aspergillus ochraceus and Aspergillus niger
carried out the reduction of the C3C4 double bond to yield
dihydrocoumarin in 24 h. Meanwhile, the first strain did not transform
dihydrocoumarin after 7 d, A. niger demonstrated to have two
divergent catabolic pathways: (a) the lactone moiety opening and further
reduction of the carboxylic acid furnishing the primary alcohol
2-(3-hydroxypropyl)phenol and, (b) the hydroxylation of the aromatic
ring of dihydrocoumarin at a specific position to give
6-hydroxy-3,4-dihydrochromen-2-one. Aspergillus flavus did not
perform double bond reductions, and only produced oxygenated
metabolites, mainly 5-hydroxycoumarin. Enzyme-specific inhibitors and a
coumarin analogous were useful to confirm the A. niger catabolic route.