3,4-dihydropyrimidin-2(1H)-ones and 1,4-dihydro-pyridines as biofilm formation promoters

C. LUCERO ESTRADA; CARLOS R. PUNGITORE; EDUARDO J. BORKOWSKI; FERNANDO SUVIRE; SATHICQ, A.G.; ROMANELLI, G.; RICARDO D. ENRIZ

Mendoza

Congreso; SBC2013; 2013

Bacteria exist in nature under two forms or states, being one of them colonies of microorganisms forming biofilms. Biofilms grants to bacteria significant advantages because provides them protection from environmental fluctuations of humidity, temperature and pH, as well as nutritious concentration, facilitating the elimination of waste and providing bigger resistance to antimicrobial agents. In this work, a group of 12 compounds formed by tetrahydropirimidines (confirmar nombre) and dihydropiridines was evaluated related with their capacity of increase the formation of biofilm by the bacteria Yersinia enterocolitica (the model selected in this occasion). The most active compound increased the observed formation of biofilm more than 5 times with regard to the control. By means of the obtained results of biological rehearsals and theoretical calculations, a mechanism was hypothesized involving the capacity of these compounds to establish covalent bonds by Michael´s type reactions, trans-esterification and nucleofilic carbonyl attack in order to biofilm formation. The reactions between the evaluated compounds and the glucuronic and mannuronic acids residues would increase the construction of the mentioned structure.