Antibacterial screening of a coumarines group

MASCOTTI L; GARRO H; PUNGITORE C; TONN C; KURINA M; GIANNINI F

San Luis

Congreso; Sociedad Biología de Cuyo; 2009

Coumarines are plant ubiquitous compounds fully distributed in families such as in Umbelliferae/Apiaceae and Rutaceae. They have been isolated as glycosides and/or in their free forms. It has been reported for them antifungal, antibacterial and, the most well known, anticoagulant activity, the last one involved in defense mechanisms against predators. The objetive of this work was to evaluate antibacterial activity of 5-hidroxycoumarin (1), 3-acylcoumarin (2), coumaranone (3), 4-hidroxycoumarin (4), coumarin (5), 3-hidroxycoumarin (6), 7-hidroxycoumarin (7), 6-hidroxycoumarin (8), against Lactobacillus acidophilus, Bifidobacterium bifidum and Bacillus subtilis, from a commercial liophilyzated of gram+ bacterum, by microdilution agar assay. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3) >250ìg/mL, 3-acylcoumarin (2) and 6-hidroxycoumarin (8) 250ìg/mL, 5-hidroxycoumarin (1) 125ìg/mL, coumarin (5) 62.5ìg/mL, 4-hidroxycoumarin (4), 7-hidroxycoumarin (7) and 3-hidroxycoumarin (6) <1.95ìg/mL. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3) >250ìg/mL, 3-acylcoumarin (2) and 6-hidroxycoumarin (8) 250ìg/mL, 5-hidroxycoumarin (1) 125ìg/mL, coumarin (5) 62.5ìg/mL, 4-hidroxycoumarin (4), 7-hidroxycoumarin (7) and 3-hidroxycoumarin (6) <1.95ìg/mL. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3) >250ìg/mL, 3-acylcoumarin (2) and 6-hidroxycoumarin (8) 250ìg/mL, 5-hidroxycoumarin (1) 125ìg/mL, coumarin (5) 62.5ìg/mL, 4-hidroxycoumarin (4), 7-hidroxycoumarin (7) and 3-hidroxycoumarin (6) <1.95ìg/mL. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. The objetive of this work was to evaluate antibacterial activity of 5-hidroxycoumarin (1), 3-acylcoumarin (2), coumaranone (3), 4-hidroxycoumarin (4), coumarin (5), 3-hidroxycoumarin (6), 7-hidroxycoumarin (7), 6-hidroxycoumarin (8), against Lactobacillus acidophilus, Bifidobacterium bifidum and Bacillus subtilis, from a commercial liophilyzated of gram+ bacterum, by microdilution agar assay. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3) >250ìg/mL, 3-acylcoumarin (2) and 6-hidroxycoumarin (8) 250ìg/mL, 5-hidroxycoumarin (1) 125ìg/mL, coumarin (5) 62.5ìg/mL, 4-hidroxycoumarin (4), 7-hidroxycoumarin (7) and 3-hidroxycoumarin (6) <1.95ìg/mL. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3) >250ìg/mL, 3-acylcoumarin (2) and 6-hidroxycoumarin (8) 250ìg/mL, 5-hidroxycoumarin (1) 125ìg/mL, coumarin (5) 62.5ìg/mL, 4-hidroxycoumarin (4), 7-hidroxycoumarin (7) and 3-hidroxycoumarin (6) <1.95ìg/mL. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3) >250ìg/mL, 3-acylcoumarin (2) and 6-hidroxycoumarin (8) 250ìg/mL, 5-hidroxycoumarin (1) 125ìg/mL, coumarin (5) 62.5ìg/mL, 4-hidroxycoumarin (4), 7-hidroxycoumarin (7) and 3-hidroxycoumarin (6) <1.95ìg/mL. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. The objetive of this work was to evaluate antibacterial activity of 5-hidroxycoumarin (1), 3-acylcoumarin (2), coumaranone (3), 4-hidroxycoumarin (4), coumarin (5), 3-hidroxycoumarin (6), 7-hidroxycoumarin (7), 6-hidroxycoumarin (8), against Lactobacillus acidophilus, Bifidobacterium bifidum and Bacillus subtilis, from a commercial liophilyzated of gram+ bacterum, by microdilution agar assay. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3) >250ìg/mL, 3-acylcoumarin (2) and 6-hidroxycoumarin (8) 250ìg/mL, 5-hidroxycoumarin (1) 125ìg/mL, coumarin (5) 62.5ìg/mL, 4-hidroxycoumarin (4), 7-hidroxycoumarin (7) and 3-hidroxycoumarin (6) <1.95ìg/mL. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3) >250ìg/mL, 3-acylcoumarin (2) and 6-hidroxycoumarin (8) 250ìg/mL, 5-hidroxycoumarin (1) 125ìg/mL, coumarin (5) 62.5ìg/mL, 4-hidroxycoumarin (4), 7-hidroxycoumarin (7) and 3-hidroxycoumarin (6) <1.95ìg/mL. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3) >250ìg/mL, 3-acylcoumarin (2) and 6-hidroxycoumarin (8) 250ìg/mL, 5-hidroxycoumarin (1) 125ìg/mL, coumarin (5) 62.5ìg/mL, 4-hidroxycoumarin (4), 7-hidroxycoumarin (7) and 3-hidroxycoumarin (6) <1.95ìg/mL. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. Umbelliferae/Apiaceae and Rutaceae. They have been isolated as glycosides and/or in their free forms. It has been reported for them antifungal, antibacterial and, the most well known, anticoagulant activity, the last one involved in defense mechanisms against predators. The objetive of this work was to evaluate antibacterial activity of 5-hidroxycoumarin (1), 3-acylcoumarin (2), coumaranone (3), 4-hidroxycoumarin (4), coumarin (5), 3-hidroxycoumarin (6), 7-hidroxycoumarin (7), 6-hidroxycoumarin (8), against Lactobacillus acidophilus, Bifidobacterium bifidum and Bacillus subtilis, from a commercial liophilyzated of gram+ bacterum, by microdilution agar assay. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3) >250ìg/mL, 3-acylcoumarin (2) and 6-hidroxycoumarin (8) 250ìg/mL, 5-hidroxycoumarin (1) 125ìg/mL, coumarin (5) 62.5ìg/mL, 4-hidroxycoumarin (4), 7-hidroxycoumarin (7) and 3-hidroxycoumarin (6) <1.95ìg/mL. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3) >250ìg/mL, 3-acylcoumarin (2) and 6-hidroxycoumarin (8) 250ìg/mL, 5-hidroxycoumarin (1) 125ìg/mL, coumarin (5) 62.5ìg/mL, 4-hidroxycoumarin (4), 7-hidroxycoumarin (7) and 3-hidroxycoumarin (6) <1.95ìg/mL. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3) >250ìg/mL, 3-acylcoumarin (2) and 6-hidroxycoumarin (8) 250ìg/mL, 5-hidroxycoumarin (1) 125ìg/mL, coumarin (5) 62.5ìg/mL, 4-hidroxycoumarin (4), 7-hidroxycoumarin (7) and 3-hidroxycoumarin (6) <1.95ìg/mL. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. It is known that pure compounds with MIC values lower than 25ìg/mL are very promising as antimicrobial agents. Therefore hidroxylated coumarines in 3, 4 and 7 positions, show interesting activities, similar to those of eritromicin and gentamicine, well known antibiotics. 1), 3-acylcoumarin (2), coumaranone (3), 4-hidroxycoumarin (4), coumarin (5), 3-hidroxycoumarin (6), 7-hidroxycoumarin (7), 6-hidroxycoumarin (8), against Lactobacillus acidophilus, Bifidobacterium bifidum and Bacillus subtilis, from a commercial liophilyzated of gram+ bacterum, by microdilution agar assay. The results obtained, expresed as MIC (minimal inhibitory concentration), are: coumaranone (3