DECOLORIZATION OF SULPHUR BLACK DYE AND REAL TEXTILE WASTEWATER BY THE ENDOPHYTIC STRAIN Talaromyces purpureogenus H4

BONILLA JO; LENCINA N; BARBERO B; KURINA SANZ M; MAGALLANES-NOGUERA C

Congreso; Congreso Argentino de Microbiología General SAIB-SAMIGE; 2021

SAMIGE

The advance of the industrial activities causes diverse contamination troubles around the world. In particular, the textile effluents represent a serious threat to the environment due to their complexity, since a great variety of commercial dyes and other substances can be found in different proportions. In recent decades, microorganisms have gained attention to be applied in bioremediation strategies. In this sense, endophytic strains are great candidates due to the promiscuity of their enzymatic systems. In a previous work, the endophytic strain Talaromyces purpureogenus H4 (GenBank MK749843.1), isolated from Handroanthus impetiginosus in Brazil, was identified. The present study aimed to screen the capacity of T. purpureogenus H4 to remove and decolorize the commercial Sulphur Black Dye 1 and a real colored textile wastewater, both obtained by courtesy of a local textile industry (San Luis, Argentina). To evaluate the dye removal capacity, a resting-cell cultivation method was applied. The microorganism was faced to 50 µg mL-1 of Sulphur Black Dye 1 in Tris-HCl 50mM buffer pH=8 during seven days at 28 °C and 160 rpm, in darkness. The decolorization was monitored by UV-VIS at 617 nm. The endophytic strain decolorization capacity was also evaluated on a real textile effluent, under the same cultivation conditions mentioned before. Additionally, physicochemical parameters stablished by the local regulations (pH, conductivity and Chemical Oxygen Demand-COD) were analyzed on the textile effluent before and after the biotreatment process. The biological catalyst showed a maximal Sulphur dye removal capacity of around 60 % at 72 h, which kept constant over time. When faced to the real effluent, the maximal microbial decolorization capacity was 75 % and it was reached at 72 h. T. purpureogenus H4 was able to decrease the effluent conductivity from 18.3 mS cm-1 to 15.7 mS cm-1. Likewise, the COD decreased from 531 mg L-1 to 263 mg L-1. The wastewater pH value (pH=8.0) was not affected by the biotreatment. Before the biotreatment, the effluent fulfilled the conditions to be discharged into sewage collectors, but not to be release into the ground or surface water bodies, according to the local regulations. After the biotreatment, the effluent parameters improved, fulfilling the conditions to be discharged directly into the ground. The results obtained in the present work invite us to in-depth into the analysis of the dye removal molecular mechanisms by studying the proteins involved in the decolorization process, as well as assessing risks and mainly toxicological aspects before the design of bioremediation protocols based on T. purpureogenus H4.