INVESTIGADORES
KURINA SANZ Marcela Beatriz
artículos
Título:
A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents
Autor/es:
RODRIGUEZ P; MAGALLANES-NOGUERA C; MENENDEZ P; ORDEN AA; GONZALES D; KURINA SANZ M; GIORDANO S
Revista:
BIOCATALYSIS AND BIOTRANSFORMATION
Editorial:
TAYLOR & FRANCIS LTD
Referencias:
Lugar: Londres; Año: 2015 vol. 33 p. 121 - 129
ISSN:
1024-2422
Resumen:
The reported anti-Prelog selectivity in Raphanus sativushairy roots prompted us to search for similar activity in other R. sativus systems, such as in vitro germinated sprouts, edible roots and its associated endophytic microorganisms.Two test substrates were used in these studies: acetophenone and rac-ethyl 2-ethyl-3-oxobutanoate. Endophytes were isolated under substrate selective pressure and individual strains were evaluated on their ability to reduce both substrates. Among the isolates, two bacteria and a fungus possessing potentiality to be used as biocatalysts were found: Bacillus megaterium that reduces acetophenone with excellent anti-Prelog selectivity, and Pseudomonas sp. and Penicillium chrysogenum with enantioselectivity and stereoselectivity for the reduction of rac-ethyl 2-ethyl-3-oxobutanoate, respectively
