Effect of TGF-beta1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells.

TM RODRIGUEZ; A SALDIAS; M IRIGO; J VELASCO ZAMORA; MJ PERONE; RICARDO A. DEWEY

Stem Cells Translational Medicine

AlphaMed Press

Año: 2015 vol. 4 p. 894 - 898

2157-6564

Adipose tissue is an attractive source of mesenchymal stromal cells (MSCs) due to the relative ease of obtaining large volumes with more MSC abundance, compared to other sources. Increasing evidence support the fact that trophic factors secreted by MSCs play a pivotal therapeutic role. Several strategies in regenerative medicine use MSCs mainly exploiting their immunosuppressive effect, and homing capacity to sites of damage. Transforming Growth Factor ß1 (TGF-ß1) is a pleiotropic cytokine that, depending on the cell niche, may display either anti-inflammatory or pro-inflammatory effects. TGF-ß1 expression increases in various tissues with damage, especially when accompanied by inflammation. Thus, we aim to analyze the effect of TGF-ß1 on the secretion by adipose-derived mesenchymal stromal cells (ASCs) of a panel of 80 cytokines/chemokines by antibody array. To avoid a possible effect of fetal bovine serum (FBS) on ASCs secretion, we performed our analysis by culturing cells in FBS-free conditions, only supplemented with 0.1% of bovine serum albumin (BSA). Here we report the cytokine profile secreted by ASCs, and that TGF-ß1 exposure modulates 8 chemokines and 18 cytokines, including TGF-ß1 and ß2, and other important cytokines involved in immunosuppression, allergic responses, and bone resorption.