LUNG AND KERATINO CYTE CELLS DIFFERENTIAL RESPONSE TO RURAL AIR PARTICULATE MATTER IN VITRO

N ORONA; F ASTORT; I FENOY; I PALAVECINO; N RAMIREZ HABERKON; M MENDEZ; A RANDI; J PANEBIANCO; A GOLDMAN

Congreso; Reunion Conjunta SAIC-SAI-AAFE-NANOMED; 2021

Atopic disorders susceptibility depends on interactions between geneticand environmental factors. The prevalence of these disordershas increased in the last 5 decades indicating that environmentalfactors might be responsible for this increase. Airborne particulatematter (PM) is one of environmental health problems affecting theworld?s population. Although several studies related the exposure tourban PM with the onset or exacerbation of allergies such as asthmaand atopic dermatitis, there are few studies that link rural PMwith the development of these allergic disorders. Rural PM comesmainly from agricultural activity, where agrochemical compoundsare frequently used. Although agrochemical exposure has beenrelated to allergic diseases, an approach considering the synergisticeffects of PM and agrochemicals is necessary. Therefore, theaim of this study was to evaluate the effect of soil generated-PM10 from an intensive cultivation area where the use of agrochemicalsprevails (Rural PM) from the central Pampas region on pulmonary(A549) and skin (HaCaT) cells. A549 or HaCaT cells were exposedto Rural PM (1-100μg/ml) or to glyphosate (0.75?7500pg/ml). After24h, cell metabolic activity, lactate dehydrogenase (LDH) and cytokine release (IL-8, TSLP, IL-1β) was assessed. We found that A549cells exposed to PM showed a marked increase in IL-8 production(P