INVESTIGADORES
GOLDMAN Alejandra
congresos y reuniones científicas
Título:
Evaluation of the immunostimulatoryproperties of plant Hsp90 to use as novel adjuvant carriers
Autor/es:
CORIGLIANO, M., GOLDMAN, A., MARTÍNV., LAGUÍABECHER, M. MAGLIOCO, A, CLEMENTE, M
Lugar:
Trelew
Reunión:
Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010
Institución organizadora:
SAIB
Resumen:
Ingeneralterms,theproductionofhighamountsofantigeninplantishard,havingtodevelopdifferentstrategiestoincreasetheirexpression.Aninterestingoptionistoexpressthepolypeptideofinterestintheplantwithacarrierthatcouldprovidestabilityandthereforeincreasethepoleptideproduction.WeconsiderthatplantHsp90couldbesuchacarrier.Inaddition,ifplantHsp90presentsadjuvantproperties,itcouldariseasanovelandinterestingcarrierforproteinsorpeptideswithimmumoprotectivevalue,improvingtheimmunogenicitypropertyofthetransgenicplantextract.WehavepreviouslydemonstratedthatrecombinantplantHsp90proteins(rpHsp90)inducedprominentproliferativeresponsesinspleencellsformBALB/cmice.Here,itwasfoundthatinvitroincubationofspleencellswithrpHsp90leadstotheexpansionofCD19-bearingpopulations,suggestingadirecteffectoftheseproteinsonBlymphocytes.Furthermore,weexaminedtheinvolvementofTLR4intherpHsp90sinductionofBcellproliferation.SpleencellsofC3H/HeJmice,whicharehyporesponsivetoLPS,alsorespondedpoorlytorpHsp90indicatingthatrpHsp90B-cellmitogenicpropertiesarethroughtheTLR4receptor.TheimmunostimulatorypropertiesofrpHsp90observedheresupporttheideathattheseproteinscouldbeexcellentcarriersforantigensandpeptidexpressedinplant.
