Differential Subcellular Localization of Members from Toxoplasma

DE-MIGUEL, N.; ECHEVERRIA, P.; SERGIO OSCAR ANGEL

EUKARYOTIC CELL

AMER SOC MICROBIOLOGY

Año: 2005 vol. 4 p. 1990 - 1997

1535-9778

database (www.toxodb.org) search, five parasites sHsps: Hsp20, Hsp21, Hsp28, Hsp29 and the previously characterized Hsp30/Bag1 were identified. As expected, they share the homologous a-crystallin domain, which is the key characteristic of sHsps proteins. However, the N-terminal segment of each protein contains unique characteristics in size and sequence. Most T.gondii sHsps are constitutively expressed in tachyzoites and fully differentiated bradyzoites, with the exception of Hsp30/Bag1. Interestingly, by subcellular localization we observed that T. gondii sHsps are located in differents compartments. Hsp20 is located at the apical end of the cell, Hsp28 is located inside the mitochondrion, Hsp29 showed a membrane associated labeling and Hsp21 appeared throughout the cytosol of the parasites. These particular differences in the immunostaining patterns suggest that their targets and functions might be different.