The role of cytoplasmic streaming in osmotic adjustment in tomato trichome cells under water stress

KEISUKE NAKATA; HIROSHI WADA,; YAYOI ONDA; R.; ERRA BALSELLS; HIROSHI NONAMI1

Matsuyama

Workshop; 2017 CIGR World Workshop in Matsuyama; 2017

Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists (JSABEES) and JSPS

ABSTRACT ? 2017 CIGRWorld Workshop in Matsuyama ? September2017The role of cytoplasmic streaming inosmotic adjustment in tomato trichome cells under water stress Keisuke Nakata*1, Hiroshi Wada2, Yayoi Onda1,Rosa Erra-Balsells3, and Hiroshi Nonami1 (1. Facultyof Agriculture, Ehime University, Matsuyama, Japan; 2. Kyushu Okinawa AgriculturalResearch Center, National Agricultural Research Organization (NARO), Chikugo,Japan; 3. Department of Organic Chemistry,University of Buenos Aires, Buenos Aires, Argentina) Abstract: Cytoplasmicstreaming occurs as myosin-linked organelles move along actin filaments byusing the energy of adenosine triphosphate formed during glycolysis, respiration,and photosynthesis to carry organelles, nutrients, metabolites, geneticinformation, and other materials to all parts of the cell. Cytoplasmic streaming velocityinside the trichome cells on the tomato fruit declines from the glandular cellsaccording to low water inflow into the fruit by ripening. Hence, the velocitylowering suggestsrelationship between cytoplasmic streaming velocity and the cell water status. Combiningthe cell pressure probe and an orbitrap mass spectrometer, the pico pressure-probe-electrospraymass spectrometry (picoPPESI-MS) system can be performed high spatial-resolutioncell sampling and water status measurement, precise volume control, and highdetection sensitivity. Turgor pressure (Øp),osmotic potential (Øs), water potential (Øw), cytoplasmicstreaming velocity, and metabolites of stalk cells of type II trichomes on theadaxial leaf surface of tomato (Solanum lycopersicum L.cv. Micro-Tom) in a growth chamber were measured before and after water stresstreatment by using picoPPESI-MS system. After the plants were subjected to the water stress, Øp,Øs, and Øw of the stalk cellsdeclined immediately, and stabilized in 30 minutes. Complete recovery of themwas observed after 12 h of the treatment. By contrast, the cytoplasmicstreaming velocity was maintained during the treatment, and some phosphatesignal intensities quantitively increased immediately after the treatment.Therefore, the picoPPESI-MS method could evaluate phosphate metabolic pathwaysof the living single cells qualitatively and quantitatively, and cytoplasmicstreaming velocity might be critical factor for osmotic adjustment.