CONICET | Buscador de Institutos y Recursos Humanos

P9 Drought Tolerance Tulip flower stalk elongation and sugar metabolisms in relation to the water status. Phsychrometer and UV-MALDI-TOF MS analysis. H. Nonami1, N. Sugimoto1, H. Wada1, M. Akita1 and R. Erra-Balsells2 1Faculty of Agriculture, Ehime University, Matsuyama, 790-8566 Japan 2Dept. of Organic Chemistry, University of Buenos Aires, 1428 - Buenos Aires, Argentina Tulip plants require low temperature to initiate the stalk elongation and flowering. However, if tulip bulbs are stored at high temperature, their stalk elongation will be inhibited. When cell solutions extracted from bulbs stored at low temperature or high temperature were analyzed with the psychrometer, it was found that cells of bulbs stored at low temperature contained much more osmotically active solutes than those stored at high temperature. Because starch is stored in scales of tulip bulbs, it is thought that most osmotically active solutes may be derived from results of starch degradation in cells of bulb scales at low temperature. We attempted mass spectrometry (MS) of cell solutions extracted directly from bulb scales to compare with results obtained with the psychrometry. Cell solutions extracted from tulip bulbs during vernalization treatments were analyzed with UV-MALDI-MS and ESI-TOF-MS. When accumulation of osmotically active solutes was detected with the psychrometer in scale cells of bulbs stored at low temperature, MS analyses revealed distinctive signals of oligosaccharides derived from starch degradation. Also, distinctive polypetides were detected in the cell solution of the scales with UV-MALDI-TOF-MS when a-cyano-4-hydroxy-cinnamic acid was used as a MALDI matrix.

enviar mensaje