STUDY OF THE VIABILITY OF TRITRICHOMONAS FETUS IN BOVINE CERVICAL FLUID

ESTEFANIA SERENO BRUNO; LUMILA FUCHS; MARIANA MORERO; JORGE OYHENART; FRANCO PARADISO; MARÍA ROSANA RAMÍREZ

Otro; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020; 2020

Bovine trichomonosis is a venereal disease caused by the protozoan Tritrichomonas foetus and is very common in countries where extensive husbandry and natural service are practiced. In the bull, the presence of the parasite is asymptomatic and tends to become chronic in the adult animal. Infection in females occurs naturally during heat and usually manifests as failed services and repetition of heat. In pregnant females, the protozoan can reach the uterus and invade the embryo tissues. The multiplication of T. foetus must guarantee its persistence until a new estrus and the infection of the male. For the reasons mentioned, in this work the ability of T. foetus to grow in vitro in cervical mucus (MCV) of females at different stages of the estrous cycle was evaluated. For this, the degree of crystallization (fern formation) and the cellularity of each cervical fluid were used in the work methodology in order to infer the moment of the estrous cycle that allows the multiplication of the protozoan. The data were analyzed by ANOVA, using the Graph Pad Prism version 5.01 program. The preliminary data obtained show that T. foetus showed positive growth during estrus. In contrast, no growth was observed in the right-hander. The results allow us to conclude that it is hypothesized that the protozoan in a natural infection must modify the environment to persist in the female reproductive tract throughout the entire estrous cycle.