Region-specific astrocytes exerts differential neuroprotection in an in vitro model of Huntington`s Disease

JULIETA SABA; LOPEZ COUSELO FEDERICO; TURATI, JUAN; CARNIGLIA, LILA; DANIELA DURAND; LASAGA, MERCEDES; CARLA CARUSO

Congreso; Reunion Anual de sociedades de biociencia; 2019

SAIC

Evidence shows that there is significant heterogeneity in astrocytes, regarding morphology, gene expression and function. In this work, we investigated the action of astrocytes from two areas involved in Huntington`sdisease (HD): the striatum and the cortex. We have previously demonstrated that BDNF exerts protective and antioxidant effects on astrocytes treated with3-nitropropionic acid (3-NP), a toxinwidely usedto model HDin vitro. First,we determined TrkB expression and ERK activation in cortical and striatal cultured primary rat astrocytes treated with BDNF (50 ng/ml)+ 3-NP (15mM) for 24h.Weshowed before thatcultured astrocytes do not express TrkB-full length (TrkB-FL) protein; therefore TrkB-truncated (T1) isoform expression was assessed by Western blot. In cortical and striatal astrocytesBDNF and BDNF+3-NP increased protein expression of TrkB-T1expression(p