Astroglial mGlu3R functions in aging astrocytes and hippocampus in a model of Alzheimer?s disease

TURATI, JUAN; DELIA RAMÍREZ; CARNIGLIA, LILA; JULIETA SABA; CARLA CARUSO; JUAN, BEAUQUIS; FLAVIA, SARAVIA; DANIELA DURAND; LASAGA, MERCEDES

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Congreso; Reunion Anual de sociedades de biociencia; 2020

Our laboratory recently showed that metabotropic glutamate receptors (mGlu3R) inastrocytes presents a strong neuroprotective action against different inflammatoryagents like NO and Aβ. Physiological cerebral aging is characterized by anincrease in oxidative stress, neuroinflammation, which among others alterationscompromise cognitive functions. As an in vitro aging model, we developed primaryastrocytes cultures of 3 weeks (3w) and 9 weeks (9w), treating them with themGlu3R agonist LY379268 (LY), 0.01µM/24hr. We found that the cells reach asenescence state at 9w (β-Galactosidase assay). Furthermore, we observeddecreased cell viability at 9w, MTT assay. We also observed an importantdecrease of SOD activity comparing 9w astrocytes to 3w, while LY treatmentinduced a significant (p