Protective effect of menhaden oil on renal necrosis occurring in wealins rats fed a lipotropic-deficient diet.

A.J. MONSERRAT; C. CARUSO; C. COURRÈGES

San Diego, California

Congreso; Experimental Biology 2000; 2000

The American Physiological Society, American Society for Investigative Pathology, American Society for Nutritional Sciences, and the American Association of Anatomists.

Weanling rats fed a diet deficient in lipotropic factors (choline, methionine, B12, folic acid), develop acute renal failure whose morphological features varies from tubular jto cortical necrosis. Experiment 1. Forty weanling Sprague-Dawley male rats were allotted to 4 different groups and fed as follows: group 1, lipotropic deficient diet (LDD) with hydrogenated vegetable oiland corn oil as lipids; group 2, LDD with menhaden oil as lipid, groups 3 and 4, similar to groups 1 and 2 plus choline. Rats were fed ad libitum until they died, surviving animals were killed on day 21. Mortality in the 4 groups was 60,0, 0 and 10% respectively. Rats from groups 2, 3 and 4 did not show renal damage. Rats from group 2, 3 and 4 did not show renal damage. Rats from group 1 showed tubular or cortical necrosis in dead ratas and reparative changes in those killed on the 21th day. Experiment 2. Similar to 1, except that 45 weanling Wistar male rats were used and they were killed on the 7th day. All rats from group 1 showed renal necrosis, no renal damage was found in rats from group 2, 3 and 4. Urea and creatinine corroborated the renal changes.