Regulation and role of Acyl-CoA Synthetase 4 in neurosteroidogenic cells

MELINA DATTILO; HERRERA, LUCIA; BENZO YANINA; JESICA PRADA; COHEN SABBAN, JUAN MANUEL; GARCIA, CORINA ILEANA; PAZ, CRISTINA; MALOBERTI, PAULA MARIANA

Congreso; LXIV Reunión anual de la sociedad argentina de investigaciones clínicas; 2019

0812 - REGULATION AND ROLE OF ACYL-COASYNTHETASE 4 IN NEUROSTEROIDOGENIC CELLSMelina Andrea DATTILO (1) | Lucía Manuela HERRERA(1) |Yanina BENZO(1) | Jesica Giselle PRADA(1) | Juan ManuelCOHEN SABBAN(1) | Corina Ileana GARCÍA(2) | Cristina PAZ(1) |Paula Mariana MALOBERTI(1)INBIOMED- INSTITUTO DE INVESTIGACIONES BIOMÉDICAS.UBA-CONICET (1); FUNDACIÓN INSTITUTO LELOIR - IIBBA (2)Abstract/Resumen: The brain is a neurosteroidogenic organ inwhich neurons, astrocytes and microglia express enzymesrelated to neurosteroidogenesis. Acyl-CoA synthetase 4 (ACSL4)enzyme expression in the brain has been described. Although therole of ACSL4 in neurons and in the development of the nervoussystem has been studied, nothing had been described about itsrole in neurosteroidogenesis and its regulation in glia cells. Herewe show that in primary astrocyte cultures incubated with 1mM8Br-cAMP for 12 h, ACSL4 mRNA levels are increased comparedto untreated cells (p