Ca2+ Channels and Synaptic Transmission at the Adult, Neonatal, and P/Q-Type Deficient Neuromuscular Junction

SILVANA NUDLER, JOAQUIN PIRIZ, FRANCISCO J URBANO, MARCELO D ROSATO-SIRI, ERIKA S PIEDRAS RENTERIA, AND OSVALDO D UCHITEL

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES.

BLACKWELL PUBLISHING

Lugar: Nueva York; Año: 2003 vol. 998 p. 11 - 17

0077-8923

Different types of voltage-activated Ca2+ channels have been established based on their molecular structure and pharmacological and biophysical properties. One of them, the P/Q type, is the main channel involved in nerve-evoked neurotransmitter release at neuromuscular junctions and the immunological target in Eaton-Lambert Syndrome. At adult neuromuscular junctions, L- and N-type Ca2+ channels become involved in transmitter release only under certain experimental or pathological conditions. In contrast, at neonatal rat neuromuscular junctions, nerve-evoked synaptic transmission depends jointly on both N- and P/Q type channels. Synaptic transmission at neuromuscular junctions of the ataxic P/Q type Ca2+ channel knockout mice is also dependent on two different types of channels. Synaptic transmission at neuromuscular junctions of the ataxic P/Q-type Ca2+ channel knockout mice is also dependent on two different types of channels, N- and R-type. At both neonatal and P/Q knockout junctions, the K+-evoked increase in miniature endplate potential frequency was not affected by N-type channel blockers, but strongly reduced by both P/Q- and R-type channel blockers. These differences could be accounted for by a differential location of the channels at the release site, being either P/Q- or R-type Ca2+ channels located closer to the release site than N-type Ca2+ channels. Thus, Ca2+ channels may be recruited to mediate neurotransmitter release where P/Q-type channels seem to be the most suited type of Ca2+ channel to mediate exocytosis at neuromuscular junctions.