Study of spermatogonia population in infertile patients with immune cells infiltrates

GONZÁLEZ, LUCAS; AMARILLA, MARIA SOFÍA; CINTHIA SOLEDAD MÉNDEZ; LEILANE GLIENKE ; HERNAN OXILIA; FULCO, MARÍA FLORENCIA; JACOBO PATRICIA VERÓNICA; SOBARZO CRISTIAN MARCELO,; MARÍA SUSANA THEAS

Mar del Plata

Congreso; LVIII Reunion Anual de la Sociedad de Investigación Clínica; 2018

SAIC

Azoospermia is linked to local testicular microenvironment deregulation, with inflammatory cells present in the 15% of testicular biopsies of infertile patients. The aim of this study was to study the kinetics behavior of spermatogonia (SP) population in infertile patients displaying testicular immune cell infiltrates. Biopsies obtained from infertile patients (Hospital. Clínicas, CABA) with obstructive and non-obstructive azoospermia were classified according to morphological criteria in: normal spermatogenesis (N, n=7), hypospermatogenesis (Hypo, n=5) and severe hypospermatogenesis (SHypo, n=5). The number of immune cells was quantified by immunohistochemistry employing the leukocyte common antigen marker (CD45) and the number of SP (A dark, A pale and B) by morphological criteria in the same slides. The number of CD45 cells increased significantly in Hypo and SHypo biopsies vs N (Mean±SEM N:22.150±2.510, Hypo:69.090±9.398, SHypo:95.180±12.54, p