Involvement of nitric oxide and TNF-ALPHA; in cell death and proliferation of GC-1 spermatogonia cell line

FERREIRO, MARIA EUGENIA ; MENDEZ, CINTHIA SOLEDAD; FERRARI,S MARÍA JIMENA ; SOBARZO, CRISTIAN MARCELO; JACOBO, PATRICIA VERÓNICA; LUSTIG, LIVIA; THEAS, MARÍA SUSANA

Mar del Plata

Congreso; LXI Reunión de la Sociedad Argentina de Investigación Clínica (SAIC); 2016

Sociedad Argentina de Investigación Clínica

Nitric oxide (NO) and Tumor Necrosis Factor alpha (TNF-α) are pleiotropic factors that display multiple biological functions. In experimental autoimmune orchitis (EAO), a rat model of chronic inflammation associated to infertility, spermatocytes die by apoptosis induced by high levels of NO and TNF-α while spermatogonia that persist in the basal compartment of the seminiferous tubulesare arrested. We propose that these factors are also involved in spermatogonia cell cycle control in EAO. To evaluate this hypothesis we explored the effect of NO and TNF-α/TNFR system on proliferation and death of the spermatogonia GC-1 cell line. DETANonoate(DETA, a NO donor) reduced GC-1 viability evaluated bythe colorimetric assay of MTS (mean±SEM, media: 0.499±0.013; DETA 2mM:0.344±0.024**; DETA 0.5mM:0.522±0.019; DETA 0.05mM:0.580±0.033 **p