INV SUPERIOR JUBILADO
SEILICOVICH Adriana
congresos y reuniones científicas
Título:
Behavioral characterization of a surgical model of menopause and hormone replacement treatments in rats
Autor/es:
M.IMSEN; F.MERINO; M.CODAGNONE; C.RACANA-NARVÁEZ; A. SEILICOVICH; A.REINES; S.ZÁRATE
Reunión:
Congreso; LXI Reunión de la Sociedad Argentina de Investigación Clínica; 2016
Resumen:
Ovarian hormone loss during natural or surgical menopause is associated with cognitive impairment, mood alterations and higher risk of age-related disorders. Increasing importance is placed on the translational validity of animal models of human menopause and hormone replacement treatments (HRT) to discern risk vs. benefit of such therapeutic interventions. In a previous work, we validated an animal model of long-term ovariectomy (OVX) and early estradiol (E2) and progesterone (P4) replacement therapy in terms of steady hormone levels within the physiological range and biological effects in peripheral hormone-responsive tissues. In the present work, we aim to characterize our model by analyzing different behavioral parameters modulated by ovarian hormonal status.To this aim, Wistar adult female rats were ovariectomized (OVX) or sham-operated (SHAM). In parallel, some OVX rats were s.c. implanted with silastic capsules containing 1mg E2 and/or 50mg P4. After 12 weeks, the animals were subjected to behavioral tests to evaluate parameters of depression, anxiety and spatial working memory. Twelve-week ovarian hormone deprivation induced depressive-like behavior and impaired spatial working memory, evaluated in the forced swimming test and the Y maze spontaneous alternation test, respectively. OVX rats also showed enhanced anxiety evaluated by the elevated plus maze test (OVX vs. SHAM, *p