INVESTIGADORES
PODESTA Ernesto Jorge
congresos y reuniones científicas
Título:
REGULATION OF LIPID METABOLISM AND ONCOGENESIS
Autor/es:
A. DUARTE; P. MALOBERTI; C. KARLES; U. ORLANDO; I. NEUMAN; F. CORNEJO MACIEL; A. SOLANO; E.J. PODESTÁ
Lugar:
Mar del Plata
Reunión:
Congreso; XLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2007
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)
Resumen:
The translocator protein (TSPO), a mitochondrial acyl-CoA thioesterase (Acot2) and an arachidonic acid (AA) preferring acyl-CoA synthetase (ACS4) are important for cholesterol transport and regulation of intramitochondrial AA levels in steroidogenic tissues. Some studies demonstrate the role of TSPO on the development of different types of cancer. The overexpression of TSPO correlates with the development of the aggressive phenotype of breast cancers, affecting cholesterol transport and cellular proliferation. It is known that ACS4 is overexpressed in hepatocarcinoma and colon cancer. In this work we studied the role of this enzyme, taking as model MCF7, a non aggressive and MDA-MB-231 a high aggressive breast cancer cell lines. The ACS4 levels expression correlate with the high cellular aggressiveness phenotype, as was previously described for TSPO levels in this cell lines. The overexpression of ACS4 produces a more aggressive cell phenotype, measured by proliferation, migration and cellular invasion. Moreover, we demonstrated that the intramitochondrial AA levels are elevated in high aggressive cell lines. The overexpression of ACS4 in several types of tumors would support ACS4 as a potentially early tumor marker. This is in agreement with recently results where ACS4 is one protein that is increased during the transition from normal to preneoplastic mammary tissue.