Melatonin treatment prevents the progression of experimental autoimmune orchitis

MÉNDEZ SC; GONZALEZ LN; GUALDONI GS; IMSEN M; AMARILLA MS; SOBARZO C; LUSTIG L; JACOBO PV; THEAS MS

Congreso; Reunión Anual Conjunta SAIC/SAI/SAFIS; 2018

SAIC/SAI/SAFIS

618. (148) MELATONIN TREATMENT PREVENTS THE PROGRESSION OF EXPERIMENTAL AUTOIMMUNE ORCHITIS IN RATS . Cinthia Soledad Mendez, Lucas Nicolás González, Gisela Soledad Gualdoni, Mercedes Imsen, María Sofía Amarilla, Cristian Sobarzo, Livia Lustig, Patricia Verónica Jacobo, María Susana Theas 1 Instituto de Investigaciones Biomédicas (UBA-CONICET), Facultad de Medicina, UBA. Testicular inflammation is frequently associated to infertility. We developed an experimental model of autoimmune orchitis (EAO) in rats. In EAO interstitial lymphomonocytes increased from focal (50- 60days, d) to severe EAO (70-80d), concomitantly with apoptosis of post-meiotic germ cells (GC) and pre-meiotic GC cycle arrest, leading to aspermatogenesis. Melatonin (MLT) is a hormone with anti-oxidant, anti-inflammatory and anti-apoptotic functions. The aim of this study was to evaluate whether MLT prevents the progression of testicular damage and inflammation of rats with focal EAO. Adult Wistar rats were immunized with testicular homogenate and adjuvants or not treated (Normal group, N). 60d after the first immunization, EAO rats were semicastrated and treated daily with MLT (10mg/ kg i.p, MLT group) or vehicle (1% methanol in saline, S Group) for 20d. Testicular histopathological analysis was performed to determine the inflammation and orchitis score (EAO=V+T, where V is a factor from 0 to 9 depending on the % of sloughed seminiferous tubules (ST) and T a factor that considers the severity of ST damage). The incidence of orchitis at 60d in the S group was 100% (7/7) and in the MLT group 89% (8/9). N group did not develop orchitis (EAO score:0.0). EAO and inflammation score was only determined in rats that developed EAO at 60d. EAO score significantly increased in RESÚMENES DE LAS COMUNICACIONES 245 the 100% (7/7) of the S group rats (mean±ESM 60d:3.071±0.820, 80d: 5.786±1.367, p618. (148) MELATONIN TREATMENT PREVENTS THE PROGRESSION OF EXPERIMENTAL AUTOIMMUNE ORCHITIS IN RATS Cinthia Soledad Mendez, Lucas Nicolás González, Gisela Soledad Gualdoni, Mercedes Imsen, María Sofía Amarilla, Cristian Sobarzo, Livia Lustig, Patricia Verónica Jacobo, María Susana Theas 1 Instituto de Investigaciones Biomédicas (UBA-CONICET), Facultad de Medicina, UBA. Testicular inflammation is frequently associated to infertility. We developed an experimental model of autoimmune orchitis (EAO) in rats. In EAO interstitial lymphomonocytes increased from focal (50- 60days, d) to severe EAO (70-80d), concomitantly with apoptosis of post-meiotic germ cells (GC) and pre-meiotic GC cycle arrest, leading to aspermatogenesis. Melatonin (MLT) is a hormone with anti-oxidant, anti-inflammatory and anti-apoptotic functions. The aim of this study was to evaluate whether MLT prevents the progression of testicular damage and inflammation of rats with focal EAO. Adult Wistar rats were immunized with testicular homogenate and adjuvants or not treated (Normal group, N). 60d after the first immunization, EAO rats were semicastrated and treated daily with MLT (10mg/ kg i.p, MLT group) or vehicle (1% methanol in saline, S Group) for 20d. Testicular histopathological analysis was performed to determine the inflammation and orchitis score (EAO=V+T, where V is a factor from 0 to 9 depending on the % of sloughed seminiferous tubules (ST) and T a factor that considers the severity of ST damage). The incidence of orchitis at 60d in the S group was 100% (7/7) and in the MLT group 89% (8/9). N group did not develop orchitis (EAO score:0.0). EAO and inflammation score was only determined in rats that developed EAO at 60d. EAO score significantly increased in the 100% (7/7) of the S group rats (mean±ESM 60d:3.071±0.820, 80d: 5.786±1.367, p< 0.05). EAO score was similar at 60 and 80 d in the MLT group (mean±ESM, 60d:3.750±0.366, 80d: 3.750±1.065).in MLT group. EAO was reduced in 65% (5/8), was similar in 12% (1/8) and was increased in 25% (2/8) of the animals. Inflammation score significantly decreased in MLT vs S group at 80 d (mean ± ESM ,S80d:2.875±0.459, MLT 80d: 1.333±0.408, N80d: 0.500±0.500, p < 0.05. We demonstrated that melatonin treatment prevents the progression of orchitis and reduces the inflammatory microenvironment.