Involvement of NO and TNF-alpha; in cell death and proliferation of GC-1 spermatogonia cell line

FERREIRO ME; MENDEZ CS; FERRARIS J; GONZALEZ C; SOBARZO CM; JACOBO PV; LUSTIG L; THEAS MS

Mar del Plata

Congreso; Reunión Anual Conjunta SAIC; 2016

SAIC

Nitric oxide (NO) and Tumor Necrosis Factor alpha (TNF-α) are pleiotropic factors that display multiple biological functions. In experimental autoimmune orchitis (EAO), a rat model of chronic inflammation associated to infertility, spermatocytes die by apoptosis induced by high levels of NO and TNF-α while spermatogonia that persist in the basal compartment of the seminiferous tubulesare arrested. We propose that these factors are also involved in spermatogonia cell cycle control in EAO. To evaluate this hypothesis we explored the effect of NO and TNF-α/TNFR system on proliferation and death of the spermatogonia GC-1 cell line. DETANonoate(DETA, a NO donor) reduced GC-1 viability evaluated bythe colorimetric assay of MTS (mean±SEM, media: 0.499±0.013; DETA 2mM:0.344±0.024**; DETA 0.5mM:0.522±0.019; DETA 0.05mM:0.580±0.033 **p