Physicochemical characterization of chronic venous ulcer exudates by ft-ir spectroscopy

CERUSICO, NICOLAS ABEL; AYBAR, JUAN PABLO; CHAVEZ JARA, ROMINA; LAZARTE MARIA DE LOS ANGELES; MALDONADO JAVIER; SESTO CABRAL M. EUGENIA; DI TOTO BLESSING LILIAN EDITH; GONZALEZ EXEQUIEL ELIAS; JUAN C VALDEZ; BEN ALTABEF AIDA; RAMOS VERNIERI, ALBERTO NICOLAS

Buenos aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad de Biociencias

Chronic venous leg ulcers (CVLU) is a complication of chronic venous insufficiency. According to clinical state, ulcer exudate presents differences in its relative composition (plasma ultrafiltrate, bacteria, inflammatory molecules and cells). To get a better diagnostic and treatment, it?s crucial the identification of parameters involved in the clinical state as bacterial infection, inflammation, biofilm formation, protein and proteases, pH, etc. This work aims to present FT-IR spectroscopy as a novel diagnostic method for detect these components. For this, it was obtained FT-IR spectra from human CVLU: exudates, isolated planktonic and biofilm bacteria and different serum and plasma controls. In mid infrared region there are several frequency ranges that correspond to specific biomolecules bonds like lipids, proteins, nucleic acids and polysaccharides. After spectral pre-processing, percentage of the mentioned biomolecules and its ratios were calculated from respective spectral area. Also peaks from spectra 2nd derivative at the same frequency regions were identified. This clinical protocol was performed in patients from dermatology service from Avellaneda Hospital and approved by regional bioethical committee. Exudates showed repetitive and characteristic spectral profiles whose areas (dimensionless units) of lipids were (13.94±5.63), proteins (33.90±6.60), nucleic acids (2.61±1.16) and polysaccharides (19.91±11.01). Exudates from acute patients have higher lipid areas than exudates from chronic patients (p