Bacterial Nanocellulose, A Natural Non-Active Pharmaceutical Ingredient for Gel Design. Finding a Natural Mosquito Repellent, A Community Experience.

GONZALEZ EXEQUIEL ELIAS; SESTO CABRAL MARIA EUGENIA

International Clinical and Medical Case Reports Journal

Salient Visionary Publication

Lugar: Wayoming; Año: 2023 vol. 18 p. 1 - 15

2832-5788

lient Visionary PublicationsInternational Clinical and Medical Case Reports Journal (ISSN: 2832-5788) | Volume 2, Issue 18 | Research Article | Open Access DOIBacterial Nanocellulose, A Natural Non-Active Pharmaceutical Ingredient for Gel Design. Finding a Natural Mosquito Repellent, A Community ExperienceMaria Eugenia Sesto Cabral*González Exequiel, Sesto Cabral Maria Eugenia* Laboratorio de Estudios Farmacéuticos y Biotecnología Farmacéutica (LEFyBiFa)-Instituto de Biotecnología Farmacéutica y Alimentaria (INBIOFAL) - CONICET-UNT - Tucumán, Argentina*Correspondence to: Maria Eugenia Sesto Cabral AbstractBacterial Nano Cellulose (BNC) is a primary metabolite synthesized inside Pseudomonas fluorecens bacterial cell. BNC has distinctive characteristics that introduces modifications in its biological and physicochemical properties. Its swelling capacity under special conditions make it an unique model for semi-solid topical pharmaceutical forms design. Cleaner production studies developed in our laboratory demonstrate that it is possible to reduce the concentration of synthetic excipients in gel formulas with the addition of small concentrations of BNC. This work shows our experience in the redesign of the base formula for semi-solid gels, its implementation on the manufacture of a natural mosquito repelent, and a joint experience with a rural school and its comunity in Amberes-Tucuman-Argentina. The primary health care center (CAPS- Comuna de Amberes) provided alarming data regarding the prevalence of dengue virus, which is endemic in the area due to its geographical location. To the natural active pharmaceutical ingredient selection, aromatic plants from the area that contain citronellol and/or limonene in high concentrations were surveyed. Cymbopogon citratus, was selected, due to its abundance in the Amberes área. Safety evaluation and persitence studies of the repellent action were carried out on 100 healthy volunteers from Amberes commune.The main objective was to design a natural mosquito repellent, suitable for all ages, sustainable, biodegradable. To ensure the success of the technological design, quality controls established by the Argentine pharmacopeia were carried out. In addition, the results of safety, organoleptic characteristics, acceptance and persistence of repellent action, were evaluated.Since there is no foreseeable risk from the use of BNC, nor other ingredient on the repelent designed is posible and representative to evaluate the safety of their ingredients simply evaluating the appearance of edema and/or erythema (exploring potencial allergies) in clinical test. This study meets the ethical and scientists standards to design, conduct, recording and reporting studies that involve the participation of human beings stipulated by the Ministry of Health of Argentina, (Resolution Nº 1490/07).The combined polymer formulation design introduced optimal characteristics in the natural mosquito repellent gel. The selection of the plant species met the desired characteristics of high concentration of terpenes from the limonene family and abundance in the study area. The pH values obtained are suitable and help to maintain skin health. The viscosity and extensibility tested in vitro are adequate, facilitating the application and persistence of the product on the skin. Studies in healthy volunteers showed absence of edema and erythema within 24 hours. No perception of skin dryness was recorded among the 100 volunteers surveyed. The persistence of the product on the skin and the repellent action was at least 3 hours in 62% of the cases. The organoleptic properties were adequate. The gel was described as soft, with a pleasant aroma, rapid absorption and a persistent refreshing sensation.The product is suitable for use as a mosquito repellent, its design is optimal for the area where the study was carried out, due to its climatic characteristics and living conditions. It is desirable to continue the studies by expanding the number of healthy volunteers and the area of influence of the Aedes aegythi mosquito in the province of Tucumán.