Human probiotic bacteria attenuate Pseudomonas aeruginosa biofilm and virulence by quorum-sensing inhibition

ALBERTO, MARÍA ROSA; GONZALEZ SILVIA NELINA; DIAZ MYRIAN ANABEL; ARENA MARIO EDUARDO

BIOFOULING

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2020

0892-7014

This work investigated chloroform extracts from culture supernatants of two human probioticbacteria, Lactobacillus casei CRL 431 and Lactobacillus acidophilus CRL 730 for the production ofvirulence factors and quorum sensing (QS) interference against three Pseudomonas aeruginosastrains. Both extracts inhibited biofilm biomass (up to 50%), biofilm metabolic activity (up to39%), the production of the enzyme elastase (up to 63%) and pyocyanin (up to 77%), anddecreased QS, without presenting any antibacterial acgivity. In addition, the chloroform extractsof both strains disrupted preformed biofilms of the three strains of P. aeruginosa analyzed (upto 40%). GC-MS analysis revealed that the major compounds detected in the bioactive extractswere four diketopiperazines. This study suggests that the metabolites of L. casei and L. acidophiluscould be a promising alternative to combat the pathogenicity of P. aeruginosa.